Activation of influenza-specific memory cytotoxic T lymphocytes by Concanavalin A stimulation
Identifieur interne : 001A77 ( Main/Exploration ); précédent : 001A76; suivant : 001A78Activation of influenza-specific memory cytotoxic T lymphocytes by Concanavalin A stimulation
Auteurs : Maya Tsotsiashvilli [Israël] ; Raphael Levi [Israël] ; Ruth Arnon [Israël] ; Gideon Berke [Israël]Source :
- Immunology Letters [ 0165-2478 ] ; 1998.
English descriptors
- Teeft :
Abstract
Abstract: Traditionally, the in vitro activation of virus-specific memory cytotoxic T lymphocytes (CTLs) has been achieved by stimulating the CTLs with antigen-presenting cells (APCs) infected with an appropriate virus or pulsed with virus-specific antigenic peptides. Here, we describe the utilization of the polyclonal activator Concanavalin A (ConA) for in vitro restimulation of memory CTLs from virus-primed mice. Using this simple method, the activation of splenocytes with ConA for 3 days (i) eliminates the need to stimulate with virus-pulsed APCs and (ii) generates CD8+ CTLs that exhibit virus specificity and MHC-restricted lytic activity similar to CTLs obtained by conventional viral restimulation. In vitro ConA stimulation of splenocytes from BALB/c mice primed with the A/Texas/77 or A/Japanese/57 strain of influenza virus and from C57L/J mice infected with the A/Texas strain, generated CTLs with specific lytic activity. Hence reactivation of memory CTLs by this method is a general phenomenon rather than a mouse or viral strain-specific one. The ConA stimulation method used here had a recall of long-term (1 year) memory CTLs that effectively lysed virally infected targets. Further ConA-stimulated effector lymphocytes from virally primed animals have been shown to recognize and subsequently lyse target cells pulsed with virus or virus-derived peptides. The ConA reactivation of specific anti-viral CTLs may facilitate (i) studying anti-viral CTL responses and (ii) identifying of viral epitopes when unknown or when appropriate viral stimulation is impossible.
Url:
DOI: 10.1016/S0165-2478(97)00135-1
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000345
- to stream Istex, to step Curation: 000345
- to stream Istex, to step Checkpoint: 000872
- to stream Main, to step Merge: 001B32
- to stream Main, to step Curation: 001A77
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Activation of influenza-specific memory cytotoxic T lymphocytes by Concanavalin A stimulation</title><author><name sortKey="Tsotsiashvilli, Maya" sort="Tsotsiashvilli, Maya" uniqKey="Tsotsiashvilli M" first="Maya" last="Tsotsiashvilli">Maya Tsotsiashvilli</name></author><author><name sortKey="Levi, Raphael" sort="Levi, Raphael" uniqKey="Levi R" first="Raphael" last="Levi">Raphael Levi</name></author><author><name sortKey="Arnon, Ruth" sort="Arnon, Ruth" uniqKey="Arnon R" first="Ruth" last="Arnon">Ruth Arnon</name></author><author><name sortKey="Berke, Gideon" sort="Berke, Gideon" uniqKey="Berke G" first="Gideon" last="Berke">Gideon Berke</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:06F474EE13DC8392CD7091E462D7EBC506180F60</idno><date when="1998" year="1998">1998</date><idno type="doi">10.1016/S0165-2478(97)00135-1</idno><idno type="url">https://api.istex.fr/ark:/67375/6H6-GDRMLRP2-C/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000345</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000345</idno><idno type="wicri:Area/Istex/Curation">000345</idno><idno type="wicri:Area/Istex/Checkpoint">000872</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000872</idno><idno type="wicri:doubleKey">0165-2478:1998:Tsotsiashvilli M:activation:of:influenza</idno><idno type="wicri:Area/Main/Merge">001B32</idno><idno type="wicri:Area/Main/Curation">001A77</idno><idno type="wicri:Area/Main/Exploration">001A77</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Activation of influenza-specific memory cytotoxic T lymphocytes by Concanavalin A stimulation</title><author><name sortKey="Tsotsiashvilli, Maya" sort="Tsotsiashvilli, Maya" uniqKey="Tsotsiashvilli M" first="Maya" last="Tsotsiashvilli">Maya Tsotsiashvilli</name><affiliation wicri:level="1"><country xml:lang="fr">Israël</country><wicri:regionArea>Department of Immunology, The Weizmann Institute of Science, Rehovot 76100</wicri:regionArea><wicri:noRegion>Rehovot 76100</wicri:noRegion></affiliation></author><author><name sortKey="Levi, Raphael" sort="Levi, Raphael" uniqKey="Levi R" first="Raphael" last="Levi">Raphael Levi</name><affiliation wicri:level="1"><country xml:lang="fr">Israël</country><wicri:regionArea>Department of Immunology, The Weizmann Institute of Science, Rehovot 76100</wicri:regionArea><wicri:noRegion>Rehovot 76100</wicri:noRegion></affiliation></author><author><name sortKey="Arnon, Ruth" sort="Arnon, Ruth" uniqKey="Arnon R" first="Ruth" last="Arnon">Ruth Arnon</name><affiliation wicri:level="1"><country xml:lang="fr">Israël</country><wicri:regionArea>Department of Immunology, The Weizmann Institute of Science, Rehovot 76100</wicri:regionArea><wicri:noRegion>Rehovot 76100</wicri:noRegion></affiliation></author><author><name sortKey="Berke, Gideon" sort="Berke, Gideon" uniqKey="Berke G" first="Gideon" last="Berke">Gideon Berke</name><affiliation wicri:level="1"><country xml:lang="fr">Israël</country><wicri:regionArea>Department of Immunology, The Weizmann Institute of Science, Rehovot 76100</wicri:regionArea><wicri:noRegion>Rehovot 76100</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Immunology Letters</title><title level="j" type="abbrev">IMLET</title><idno type="ISSN">0165-2478</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1998">1998</date><biblScope unit="volume">60</biblScope><biblScope unit="issue">2–3</biblScope><biblScope unit="page" from="89">89</biblScope><biblScope unit="page" to="95">95</biblScope></imprint><idno type="ISSN">0165-2478</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0165-2478</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Assay</term><term>Cona</term><term>Cona stimulation</term><term>Ctls</term><term>Effector</term><term>Epitope</term><term>Immunol</term><term>Immunology</term><term>Immunology letters</term><term>Lymphocyte</term><term>Lyse</term><term>Lysed</term><term>Lysis</term><term>Lytic</term><term>Lytic activity</term><term>Memory ctls</term><term>Nucleoprotein</term><term>Peptide</term><term>Restimulation</term><term>Splenocytes</term><term>Stimulator cells</term><term>Target cells</term><term>Viral</term><term>Viral infection</term><term>Virally</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Traditionally, the in vitro activation of virus-specific memory cytotoxic T lymphocytes (CTLs) has been achieved by stimulating the CTLs with antigen-presenting cells (APCs) infected with an appropriate virus or pulsed with virus-specific antigenic peptides. Here, we describe the utilization of the polyclonal activator Concanavalin A (ConA) for in vitro restimulation of memory CTLs from virus-primed mice. Using this simple method, the activation of splenocytes with ConA for 3 days (i) eliminates the need to stimulate with virus-pulsed APCs and (ii) generates CD8+ CTLs that exhibit virus specificity and MHC-restricted lytic activity similar to CTLs obtained by conventional viral restimulation. In vitro ConA stimulation of splenocytes from BALB/c mice primed with the A/Texas/77 or A/Japanese/57 strain of influenza virus and from C57L/J mice infected with the A/Texas strain, generated CTLs with specific lytic activity. Hence reactivation of memory CTLs by this method is a general phenomenon rather than a mouse or viral strain-specific one. The ConA stimulation method used here had a recall of long-term (1 year) memory CTLs that effectively lysed virally infected targets. Further ConA-stimulated effector lymphocytes from virally primed animals have been shown to recognize and subsequently lyse target cells pulsed with virus or virus-derived peptides. The ConA reactivation of specific anti-viral CTLs may facilitate (i) studying anti-viral CTL responses and (ii) identifying of viral epitopes when unknown or when appropriate viral stimulation is impossible.</div></front></TEI><affiliations><list><country><li>Israël</li></country></list><tree><country name="Israël"><noRegion><name sortKey="Tsotsiashvilli, Maya" sort="Tsotsiashvilli, Maya" uniqKey="Tsotsiashvilli M" first="Maya" last="Tsotsiashvilli">Maya Tsotsiashvilli</name></noRegion><name sortKey="Arnon, Ruth" sort="Arnon, Ruth" uniqKey="Arnon R" first="Ruth" last="Arnon">Ruth Arnon</name><name sortKey="Berke, Gideon" sort="Berke, Gideon" uniqKey="Berke G" first="Gideon" last="Berke">Gideon Berke</name><name sortKey="Levi, Raphael" sort="Levi, Raphael" uniqKey="Levi R" first="Raphael" last="Levi">Raphael Levi</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/H2N2V1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001A77 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001A77 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= H2N2V1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:06F474EE13DC8392CD7091E462D7EBC506180F60
|texte= Activation of influenza-specific memory cytotoxic T lymphocytes by Concanavalin A stimulation
}}
| This area was generated with Dilib version V0.6.33. |  |